One Step PrimeScript III RT-qPCR Mix, with UNG, is a dedicated reagent for one-step, real-time, probe-based RT-qPCR (using the 5ʼ nuclease method). This 2X premix does not freeze at its storage temperature of -20℃, so a reaction can be started simply by adding the template sample, primer, and a probe for detecting the desired target. The quick and simple protocol allows the reverse transcription and qPCR reactions to be performed in the same tube.
